Overview

This multicenter, prospective, single-arm observational study evaluated the clinical performance of the Bioperio® protocol, a standardized, patient-centered framework for managing periodontitis. The protocol combined professional supra-gingival scaling, oral hygiene instruction and motivation, full-mouth subgingival instrumentation, and-in Stage III/IV cases-adjunctive enamel matrix derivative application in deep pockets.

Eligibility

Inclusion Criteria:

• Confirmed diagnosis of periodontitis (proximal attachment loss in ≥2 non-adjacent teeth)
• No previous treatment for periodontitis
• Systemically healthy or with well-controlled chronic medical conditions
• Willingness to participate in the study and comply with the follow-up schedule

Exclusion Criteria:

• Pregnancy or breastfeeding
• Patients previously treated for periodontitis
• Poorly controlled systemic conditions
• Refusal to participate
• Patients who did not complete the proposed treatment plan