Overview
The primary research objective of the project is to determine the role of eGC in microvascular reactivity in patients with coronary heart disease (CHD) before and after bypass grafting (cross-sectional study 2).The main objective of the project is to increase the excellence and interdisciplinarity of scientific work at MEFOS by connecting researchers from different scientific fields, improving conditions and resources for scientific work, and increasing international cooperation, in line with strategic objective 1: raising scientific excellence. The results of the project could provide pathophysiological insight into the development and maintenance of endothelial dysfunction in CVD, as well as identify new biomarkers for CVD.
Description
The primary objective of this project is to determine the association between endothelial glycocalyx (eGC) and myocardial revascularization in patients undergoing coronary artery bypass grafting and clinical outcomes of treatment (observational prospective study 2).
Specific objective 2.1: Measure eGC thickness in coronary artery disease patients before and after surgery.
Specific objective 2.2: Determine the reactivity of the bypass graft sample in in vitro experiments.
Specific objective 2.3: Quantify soluble markers of eGC, oxidative stress, and inflammation from serum and PBMC samples, as well as antioxidant capacity.
Specific objective 2.4: Determine the association between the results of functional tests, eGC, and markers from specific objective 2.3 with clinical outcomes of treatment.
Eligibility
Inclusion Criteria:
- adult patients of both sexes over 18 years of age
- Caucasian
- undergoing elective coronary artery bypass grafting (CABG)
- provided written informed consent
Exclusion Criteria:
- emergency surgery
- sepsis or severe systemic inflammation before surgery
- severe renal or hepatic insufficiency
- immunosuppression
- serious comorbidities (e.g., active cancer)
- therapies that could affect oxidative stress (e.g., high doses of corticosteroids)
