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IVF Outcomes With Time-Lapse Culture: Comparison Between PPOS and GnRH Antagonist Protocols

IVF Outcomes With Time-Lapse Culture: Comparison Between PPOS and GnRH Antagonist Protocols

Recruiting
20-45 years
Female
Phase N/A

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Overview

The goal of this clinical trial is to compare two ovarian stimulation protocols used in in vitro fertilization (IVF): the fixed Progestin-Primed Ovarian Stimulation (PPOS) protocol and the GnRH Antagonist protocol. The study will evaluate IVF outcomes and embryo development patterns using time-lapse embryo monitoring technology. The main questions the study aims to answer are:

  • Does the fixed - PPOS protocol achieve similar numbers of mature eggs, good-quality embryos, and clinical outcomes compared to the GnRH Antagonist protocol?
  • Are there differences in embryo development patterns (morphokinetics) between the two protocols when monitored by time-lapse imaging?
  • How does embryo quality (KIDScore) compare between the two protocols?

Study Design:

Researchers will randomly assign 148 women undergoing IVF to two groups:

  • PPOS group (n=74): Will receive FSH injections (oral Duphaston 20mg daily) starting from day 2-3 of the menstrual cycle
  • GnRH Antagonist group (n=74): Will receive FSH injections (Orgalutran 0.25mg injections) when follicles reach ≥14mm

Participants will:

  • Undergo controlled ovarian stimulation with their assigned protocol for approximately 10-12 days
  • Have regular ultrasound monitoring and blood tests to track follicle development
  • Undergo egg retrieval procedure when follicles are mature
  • Have all embryos cultured in the EmbryoScope time-lapse incubator with continuous monitoring
  • Have embryos frozen on Day 5/6 for future transfer

Study Location:

Department of Assisted Reproduction and Andrology, Hanoi Obstetrics and Gynecology Hospital

Study Duration: January 2026 - June 2028

Eligibility

Inclusion Criteria:

  • Women aged 20-45 years
  • Indicated for in vitro fertilization (IVF) treatment
  • Planned freeze-all embryo strategy
  • Voluntary participation in research.

Exclusion Criteria:

  • Systemic diseases
  • Use of hormonal medications within 3 months prior to enrollment
  • Oocyte donation cycles
  • Unwilling or unable to participate in research

Study details
    Infertility (IVF Patients)
    Embryo Morphokinetics
    Progestins Primed Ovarian Stimulation

NCT07409493

Hanoi Medical University

26 February 2026

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