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Circulating Tumor DNA in High Risk Localized Prostate Cancer

Circulating Tumor DNA in High Risk Localized Prostate Cancer

Recruiting
18 years and older
Male
Phase N/A

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Overview

This prospective, non-therapeutic translational biomarker study will collect blood in patients with high risk localized prostate cancer prior to prostatectomy.

Description

The Vogelstein lab has developed a highly sensitive, tumor-informed method of detecting circulating free DNA (cfDNA) shed by solid tumors. Plasma will be assayed for ctDNA using the SaferSeqS tumor-informed assay, employing DNA sequences derived from prostatectomy specimens. The abundance and molecular characteristics of ctDNA will be evaluated for a pilot group of 12-24 patients using an adaptive statistical design.

Eligibility

Inclusion Criteria:

  1. Must have histologically confirmed prostate cancer.
  2. Age ≥ 18 years.
  3. ECOG performance status of 0-1.
  4. Must have the ability to understand and the willingness to sign a written informed consent document.
  5. Willing to provide serial blood samples for the study.
  6. Willing to provide tumor tissue (prostatectomy for primary cohort; prostatectomy or biopsy for exploratory cohort) for correlative studies which will compare ctDNA to tumor specimens.
  7. Primary Cohort: High-risk localized prostate adenocarcinoma defined as one or more of the following:
    • Clinical stage ≥ cT3a, Grade Group 4 or 5 (Gleason sum 8-10), and PSA ≥ 20
     *Non-bulky pelvic lymphadenopathy and indeterminate findings on staging imaging (CT,
     bone scan, PSMA PET CT) are allowed if the surgeon believes RP is appropriate.

8. Exploratory Cohort: Men with a diagnosis of prostate adenocarcinoma and one of the

following
  • Localized prostate adenocarcinoma on active surveillance
  • Biochemically-recurrent prostate adenocarcinoma after definitive local therapy
  • Hormone-sensitive, metastatic prostate adenocarcinoma
  • Metastatic CRPC

Exclusion Criteria:

  1. History of another primary cancer within the last 3 years, except for non-melanomatous skin cancer.
  2. Receiving androgen deprivation or other systemic therapy for prostate cancer.
  3. Medical condition or social situation that may preclude adherence to the protocol.

    -

Study details
    Prostate Cancer

NCT07222436

University of Pittsburgh

1 November 2025

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