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Comparison of Two-Position and Four-Position Cervical Injection Techniques for Sentinel Lymph Node Mapping in Endometrial Cancer Using Methylene Blue

Comparison of Two-Position and Four-Position Cervical Injection Techniques for Sentinel Lymph Node Mapping in Endometrial Cancer Using Methylene Blue

Recruiting
18-75 years
Female
Phase N/A

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Overview

This clinical trial evaluates lymph node mapping in newly diagnosed endometrial cancer patients undergoing surgery. The standard technique uses a 2-point methylene blue cervical injection. The study aims to determine if increasing injection points improves mapping success.

Description

In recent years, Sentinel Lymph Node (SLN) mapping has become a key focus in improving surgical outcomes for endometrial cancer. While methylene blue is a cost-effective option for SLN mapping, its detection rates remain suboptimal. This study aims to evaluate whether increasing the injection points from the standard 2-point to a 4-point methylene blue injection improves SLN mapping success.

This randomized controlled trial will include patients with preoperative stage I endometrial carcinoma. Participants will be randomized into two groups: one group will receive a 2-point methylene blue cervical injection, and the other group will receive a 4-point injection.

The primary endpoint is the difference in SLN detection rates between the two techniques. Findings from this study will provide detailed insights into whether the 4-point injection offers a significant advantage over the standard 2-point method.

Eligibility

Inclusion Criteria:

  • Individuals diagnosed with stage I endometrial cancer based on CT and PET-CT imaging.
  • Individuals with a pathologically confirmed diagnosis of stage I endometrial cancer.

Exclusion Criteria:

Medical Conditions

  • Individuals diagnosed with dementia.
  • Individuals with allergies to methylene blue or iodine.
  • Individuals who have received active treatment for another malignancy within the past five years.
  • Inability to successfully perform PLD (pelvic lymphadenectomy) or history of prior PLD.
  • Women with multiple and confluent lymph nodes identified as positive on FDG-PET/CT (such cases are not considered stage I).

Cancer-Related Conditions

  • Individuals with clinically or radiologically identified positive lymph nodes or metastatic disease.
  • Individuals with a history of pelvic dissection and/or radiation therapy.
  • Individuals with advanced cervical or uterine cancer.
  • Individuals with T3/T4 lesions.
  • Individuals with cervical tumors larger than 2 cm.

Organ Dysfunction

• Individuals with hepatic dysfunction and a MELD score ≥ 10 and creatinine ≥ 2·0 mg/dl patients.

Study details
    Endometrial Neoplasms
    Endometrial Cancer Stage I
    Sentinel Lymph Node
    Metastasis

NCT07040657

Dokuz Eylul University

16 October 2025

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