Overview
This is a randomized observational study. The main aim is to determine potential oocyte competence predictive mRNA expression profiles in the cumulus cells isolated form individual oocytes. In 2 patient cohorts: 1)GnRH Antagonist & hr-FSH with GnRH Agonist trigger, 2) Progesterone & hr-FSH with GnRH Agonist trigger.
Eligibility
Inclusion Criteria Donor:
- Patients are 18 to 35 years old
- BMI between 17-30
- Regular menstrual cycles
- AFC > 8
- Patient profile in compliance with SEF (Sociedad Española de Fertilidad) directives for egg donation (medical, psychological and genetic screening, informed consent)
- Patients' stimulation: GnRH Antagonist & hr-FSH with GnRH Agonist trigger or Progesterone & hr-FSH with GnRH Agonist trigger
- Patients agree that the oocytes will be denuded for cumulus testing and provide written informed consent for participation to the study
Exclusion Criteria Donor:
- BMI < 17 or > 30
- Extreme irregular menstrual cycles (<20 days or >40 days)
- AFC < 8
- <8 MII on previous egg retrieval
- Women with history of poor oocyte maturation or known maturation defect or unexplained failure in previous treatments
- Patients that fail to comply with SEF (Sociedad Española de Fertilidad) directives for egg donation (medical, psychological and genetic screening, informed consent)
Inclusion Criteria Recipient:
- Patients applying for ART egg donation with fresh/frozen sperm from the partner or frozen donor sperm. With eSB-FET in modified natural cycle or an HRT cycle.
- Patients are from 18 to 50 years old.
- Patients will be treated by ICSI (intracytoplasmic sperm injection) and eSB-FET (elective Single Blastocyst Frozen Embryo Transfer)
- Recipient patients agree that the donors' oocytes will be denuded for cumulus testing and provide written informed consent for participation to the study
Exclusion Criteria for oocyte Recipients
- Patient included in any other prospective study.
- BMI < 17 or > 35
- Severe uterine factor: Multiple myomectomy, multiple fibroids, major uterine malformation (unicorn, septum), Asherman Sd, severe adenomyosis
- Repeated Implantation Failure or Repeated Pregnancy Loss after euploid Single Blastocyst Transfer
- Severe male factor: Abnormal Caryotype or FISH, severe OAT / Cripto-Azoospermia, DNA Fragmentation >50% after medical treatment
- TESE / TESA (testicular sperm extraction/aspiration)