Description

Cancer is a global health issue that is on an increasing trend in terms of incidence and mortality rates, hindering the increase in life expectancy. According to the World Health Organization, lung, colorectal, and liver cancers are among the most common causes of cancer-related deaths. In Turkey, the incidence and mortality rates of lung cancer are higher than the world average. are among the risk factors that may increase the risk of lung cancer. In addition to risk factors like family history, smoking, different studies have shown that dysbiotic oral microbiome may contribute to the risk of lung cancer.

The oral microbiome is the second most diverse microenvironment in our body and has been associated with many diseases, including lung cancer. Studies to date on lung cancer-oral microbiome have generally involved designs aimed at resolving cause-and-effect relationships through statistical differences and/or mechanisms involving microbiome units.

However, there is no literature on any study aimed at developing a deep learning-based diagnostic method that focuses on the oral microbiome.Therefore, the proposed study aims to develop a microbiome based deep learning diagnostic method for lung cancer diagnosis. To this end, an observation cohort will be established consisting of 192 lung cancer patients, 192 non-cancerous benign lung disease patients, and 192 healthy controls. Tongue swab samples belonging to the cohort will be collected, and 16S rRNA sequencing will be performed. At the same time, an international observation cohort of approximately 1700 individuals will be created using in silico data. All data will be analyzed integratively, and an artificial intelligence diagnostic algorithm that can differentiate lung cancer patients from other lung diseases and healthy individuals will be developed using deep learning methods. In the final stage, the performance of the diagnostic method developed for a pilot clinical trial cohort of 96 individuals will be tested using a PRoBE (prospective specimen collection before outcome ascertainment and retrospective blinded evaluation) design.

The original aspects of the project are the proposal of a novel design in the literature, the creation of an experimental design/clinical trial and the presentation of a potential solution proposal that may have a high impact on an important diagnostic problem.

If the project is successfully completed, an artificial intelligence-based method that can potentially diagnose lung cancer through non-invasive oral microbiome samples will be developed. In addition to its patentability, if the method is further developed (in the medium to long term) into a product, it will enable lung cancer screening to be easily performed even in primary healthcare institutions with a simple oral swab sample.