Overview

This phase II trial evaluates Tunlametinib (MEK inhibitor) ± PD-1 in NRAS-mutant advanced thyroid cancer.

Key Objectives:

Assess efficacy (ORR by RECIST v1.1) Evaluate safety profiles

Study Design:

Single-arm, single-center 4 cohorts based on:

• Histology (differentiated vs. poorly/undifferentiated)
• Prior therapy status

  Treatment

• Cohorts 1-2: Tunlametinib monotherapy (12mg BID)
• Cohorts 3-4: Tunlametinib + PD-1 (commercially available)

Key Procedures:

Screening: NRAS testing + full staging (CT/MRI/PET) Monitoring: q3-week labs, q9-week imaging Follow-up: 30-day safety visit + q3-month survival tracking

Endpoints

Primary: ORR Secondary: Safety (CTCAE), PFS, DoR

Unique Aspects:

First study targeting NRAS in thyroid cancer with MEK+PD-1 Includes rare aggressive subtypes (poorly/undifferentiated)

Eligibility

Inclusion Criteria:

1. Patients voluntarily join this study and sign an informed consent form;
2. Age: ≥ 18 years old, male or female not limited;
3. For locally advanced or recurrent/metastatic thyroid cancer diagnosed by histology and unable to undergo curative surgery, one of the following requirements must be

   met

   1. Iodine refractory thyroid cancer;
   2. Differentiated thyroid cancer not suitable for iodine therapy;
   3. High grade, poorly differentiated or poorly differentiated thyroid cancer;
   4. Undifferentiated thyroid carcinoma;

4. Have undergone NGS testing (at our hospital or an external hospital), and be able to

   find that the test report or medical history has recorded a positive result for NRAS mutation in the gene test.

5. ECOG score 0-2 points; According to RECIST v1.1 evaluation, there is at least one assessable lesion;
6. Expected survival>12 weeks; 8. The main organ function and bone marrow function are normal.
   • Blood routine: Hemoglobin ≥ 90 g/L (no blood transfusion within 14 days); Absolute neutrophil count ≥ 1.5 × 109/L; platelet count ≥ 100 × 109/L;
   • Liver function: Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) are ≤ 2.5 times the upper limit of normal (ULN). If there is liver metastasis, ALT and AST are ≤ 5 times ULN, and ALP is ≤ 6 times ULN; Total bilirubin ≤ 1.5 × ULN; Albumin ≥ 30 g/L;
   • Renal function: serum creatinine ≤ 1.5 × ULN or creatinine clearance rate calculated by Cockroft Gault formula>60 mL/min;
   • Cardiac function: Echocardiography shows a left ventricular ejection fraction (LVEF) of ≥ 55%; ECG QTcF ≤ 480ms; Creatine kinase (CK) ≤ 1 × ULN, troponin/hypersensitive troponin ≤ 1 × ULN;
   • Coagulation function: International normalized ratio of prothrombin time (INR) ≤ 1.5 × ULN; Activated partial thromboplastin time (APTT) ≤ 1.5 × ULN; 9. Women of childbearing age must undergo a pregnancy test (serum or urine) with a negative result within 14 days before enrollment, and voluntarily use appropriate contraception methods during the observation period and within 3 months after the last administration of the study drug; For males, surgical sterilization or consent to use appropriate contraception methods during the observation period and within 3 months after the last administration of the study drug should be used.

Exclusion Criteria:

1. Within 4 weeks before the first medication, major surgery (excluding biopsy and outpatient minor surgery, such as placement of vascular access) or severe trauma occurred;
2. There are clinical symptoms of third interstitial fluid accumulation that cannot be controlled by drainage or other methods (such as a large amount of pleural fluid or ascites);
3. Cardiovascular and cerebrovascular diseases with impaired heart function or significant clinical significance, including but not limited to any of the

   following

     Within 6 months prior to the start of treatment, acute coronary syndrome occurred,
     including acute myocardial infarction, unstable angina, coronary artery bypass
     grafting, coronary angioplasty, and stent implantation;
       -  Symptomatic congestive heart failure (NYHA classification ≥ II); Evidence of
          clinically significant arrhythmia and/or conduction abnormalities within 6
          months prior to the start of treatment or at present;
       -  Poor control of hypertension (systolic blood pressure ≥ 140 and/or diastolic
          blood pressure ≥ 90 mmHg under medication control); Abnormal cardiac valve
          morphology (≥ grade 2) recorded by echocardiography, Note: Patients with grade
          1 abnormal valve morphology (such as mild regurgitation/stenosis) are allowed
          to be enrolled, but patients with moderate valve thickening are prohibited from
          being enrolled;
       -  History of congenital long QT syndrome; Or those who take drugs known to
          prolong the QT interval and cannot guarantee discontinuation during the study
          period;

4. Previous or screening retinal diseases, such as retinal vein occlusion (RVO),

     retinal artery occlusion, retinal vasculitis, diabetes retinopathy, hypertensive
     retinopathy, retinal telangiectasia (Costs disease), retinal pigment epithelial
     detachment (RPED), etc; Risk factors for RVO during screening (such as uncontrolled
     glaucoma or high intraocular pressure, history of high viscosity or
     hypercoagulability syndrome); Retinal diseases such as RPED;

5. Patients with interstitial lung disease or interstitial pneumonia, including

     clinically significant radiation pneumonitis (i.e. affecting daily living activities
     or requiring intervention treatment);

6. Positive for human immunodeficiency virus (HIV) antibodies, syphilis antibodies

     (Anti TP), hepatitis C virus (HCV) antibodies and HCV RNA, hepatitis B virus surface
     antigen (HBsAg) and HBV DNA (HBsAg positivity requires further testing for HBV DNA,
     HBV DNA ≥ 200 IU/ml, or ≥ 103 copies/ml);

7. History of allogeneic bone marrow transplantation or organ transplantation;

8. There are uncontrollable active infectious diseases (such as intravenous drip of

     antibiotics, antifungal or antiviral drugs) within 2 weeks before the first
     administration, or fever of unknown cause>38.5 ° C occurs during screening/before
     the first administration;

9. Irreversible electrolyte abnormalities (hypokalemia, hypomagnesemia, hypocalcemia

detected by blood biochemistry);

10. Neuromuscular diseases related to CK elevation in the past or present (such as

     inflammatory myopathy, muscular dystrophy, amyotrophic lateral sclerosis, spinal
     muscular atrophy, rhabdomyolysis syndrome);

11. Arterial/venous thrombotic events that occur within 6 months prior to the first use

     of medication, such as cerebrovascular accidents (including temporary ischemic
     attacks, cerebral hemorrhage, cerebral infarction), deep vein thrombosis, and
     pulmonary embolism;

12. Within 4 weeks prior to the first use of medication, the occurrence of Grade 3

     bleeding symptoms as defined in the National Cancer Institute's Common Terminology
     Criteria for Adverse Events (NCI CTCAE v5.0);

13. Patients with a history of other malignant tumors within the past 5 years, except

     for those who have been completely cured of basal cell carcinoma of the skin,
     squamous cell carcinoma of the skin, and cervical carcinoma in situ, and/or any
     malignant tumor patients who have been cured without disease or have had no disease
     for at least 5 consecutive years;

14. Individuals who are known to be allergic to any component of the investigational

drug;

15. Have a clear history of neurological or mental disorders, including epilepsy and

dementia;

16. Central nervous system (CNS) metastasis;

17. Prior to drug administration, all relevant anti-tumor treatment toxicity reactions

     (excluding hair loss, skin pigmentation, grade 1 chemotherapy related peripheral
     neurotoxicity, grade 2 toxicity caused by immune checkpoint inhibitors such as
     elevated blood glucose or hypothyroidism) did not recover to ≤ grade 1 levels (as
     determined by NCI CTCAE v5.0);

18. Uncontrolled concomitant diseases, including but not limited to severe diabetes

     (fasting blood glucose>250 mg/dl or 13.9 mmol/L), or other serious diseases
     requiring systemic treatment;

19. Vaccination with live or attenuated vaccine within 4 weeks prior to the first dose

     (note: if enrolled, subjects must not receive live vaccine during the study
     treatment period and within 30 days after the last dose of the study drug);

20. For premenopausal female subjects (postmenopausal female patients must have been

     menopausal for at least 12 months to be considered infertile), the pregnancy test
     result is positive; During the study period and at least 30 days after the last
     administration of the study drug, reproductive age subjects (including male
     subjects' reproductive age female spouses) who are expected to become pregnant,
     breastfeed, or unwilling to use effective contraceptive measures;

21. Patients who are known to be allergic to the components of the investigational drug

or its analogues;

22. Patients who are receiving intravenous or oral medications that affect CYP

     isoenzymes (strong inducers and inhibitors of CYP2C9 and CYP3A4) and cannot
     discontinue the protocol at least one week before starting study treatment and
     during the study period; I am currently taking drugs with a narrow therapeutic
     window that are metabolized by CYP1A2;

23. Inability to swallow capsules or intractable nausea and vomiting, malabsorption,

     extracorporeal bile diversion, or any significant small bowel resection that may
     hinder the full absorption of the study drug;

24. Other situations that researchers consider unsuitable for inclusion. If accompanied

     by family or social factors, it may affect the safety of the subjects or the
     collection of data and samples.