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Effect of a Casein and Gluten Free Diet in Mexican Children With Autism Spectrum Disorder

Effect of a Casein and Gluten Free Diet in Mexican Children With Autism Spectrum Disorder

Recruiting
4-10 years
All
Phase N/A

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Overview

Assess the effect of a casein-free and gluten-free diet on gastrointestinal symptoms, clinical variables, and the composition of the intestinal microbiota in Mexican children with autism spectrum disorder.

Description

The objective of this quasi-experimental pre-post study with time series is to evaluate the effect of a casein-free and gluten-free diet on gastrointestinal symptoms, clinical variables, and the composition of the intestinal microbiota in Mexican children with autism spectrum disorder. The main question it aims to answer is:

Does a casein free and gluten free diet promote a reduction in gastrointestinal symptoms, an improvement in clinical variables, and a state of eubiosis in the composition of the intestinal microbiota in Mexican children with autism spectrum disorder?

Participants will undergo:

An intervention of a casein-free and gluten-free diet for 3 months. Sample collection before and after the nutritional intervention. Clinical evaluations.

Eligibility

Inclusion Criteria:

Children between 4-10 years old diagnosed with autism spectrum disorder using the criteria established in the ADOS scale and the CARS.

Exclusion Criteria:

Children who have received:

  • Antibiotics
  • Antimycotics
  • Probiotic supplements
  • Prebiotic supplements
  • Any medication that may alter the microbial composition of the patient, minimum one month prior to sampling.
  • Children on a strict dietary regimen other than a casein-free and gluten-free diet.
  • Children with celiac disease or who present any gluten-related disorder.
  • Children with allergy to dairy products.

Study details
    Autism Spectrum Disorder

NCT06556290

University of Guadalajara

14 October 2025

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