Overview
The Aim of the trial:
Assess if a consecutive second ejaculate contains an equal, or even higher number of motile sperm and produces high-quality sperm for intracytoplasmic sperm injection (ICSI) in male factor patients and if it will improve clinical outcomes, such as fertilization rate, embryo quality, number of blastocysts retrieved per cycle, implantation, and hopefully pregnancy rates.
Description
- Background
According to research, the length of abstinence has an impact on sperm DNA fragmentation (SDF) as well as sperm parameters. Long abstinence period increases the volume of semen and the concentration of sperm, but it will negatively impact motility, viability, and morphology.
The second ejaculation of males shows a considerable improvement in total motility and a significant decrease in (SDF) when it is collected within 1 to 3 hours of the first ejaculate.
it is essential to ensure the availability of high-quality spermatozoa with second ejaculation to enhance clinical results in ICSI cycles
Eligibility
Inclusion Criteria:
- Male: with abnormal semen parameter according to WHO.
- Age range between 25-45 for both partners.
Exclusion Criteria:
- Female with factors that might affect Oocyte quality:
Polycystic ovary (PCO). Poor ovarian reserve. Endometriosis.
- Male partners who need testicular biopsy to retrieve sperms.