Overview

Brief Summary: * A short description of the clinical study, including a brief statement of the clinical study's hypothesis, written in language intended for the lay public.

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Severe forms of sickle cell syndrome are characterized by the occurrence of repeated vaso-occlusive crises (CVO), early complications and a high morbidity and mortality in these patients. Intensified management is then required, with the introduction of hydroxyurea treatment and then, if it proves ineffective, a transfusion program or even a haematopoietic stem cell allograft. These latter treatments present significant risks of adverse effects for the patient (haemochromatosis, erythrocyte alloimmunisation for the transfusion program, risk of GVH, chemotherapy-related toxicity, MVO for the allograft).

Hydroxyurea (HU) is the first treatment based on the specific pathophysiology of sickle cell disease. It is the first line of therapeutic intensification for adult patients and children (age ≥ 2 years) with major sickle cell disease. By mainly increasing the percentage of fetal haemoglobin (HbF), HU decreases the frequency of CVO, complications, hospitalizations and prolongs the life expectancy of patients.

The initial dose of HU, recommended by the ANSM, is 15 mg/kg/d once daily. However, the optimal dose cannot be predicted at the start of treatment, which is why a dosage adjustment is essential. The usual dose is between 15 and 35 mg/kg per day.

Typically, the dose is increased every 3 months until a mild myelosuppression tolerated by the patient is reached, indicating that the maximum tolerated dose (MTD) has been reached. When the dose of HU has reached the MTD, the ratio of clinical (reduced frequency of vaso-occlusive attacks) and biological (better % of HbF) benefits to risk (toxicity) is optimal for the patient.

The disadvantages of this practice are that:

• dose escalation can be long (9-12 months)
• clinicians may be reluctant to escalate HU to MTD
• patients are treated sub-optimally during the therapeutic adaptation period.

Recent work has shown that it is beneficial for the patient to adjust the initial dose using a pharmacological therapeutic approach in addition to monitoring haematological tolerance.

Thus, by customizing the dose of HU using an area under the curve (AUC) measurement at the initial intake of HU at a standardized dose (20 mg/kg/day), the MTD would be achieved in a faster time frame of 6-9 months.

The primary objective of our trial is to identify the methodology that will most effectively decrease the time to reach the MTD (therapeutic target). The immediate benefit will be a reduction in CVO which is the major clinical problem and leads to a risk of complications in sickle cell disease.

Eligibility

Inclusion criteria:

• Subjects aged between 2 and 35 years.
• For very young children hydroxyurea is only initiated in cases of severe sickle cell disease.
• Sickle cell genotype: HbSS
• Subjects who have been hospitalised for CVO in the last 3 months and for whom HU treatment is to be initiated and/or whose treatment is not balanced or is less than 30 mg/kg, regardless of the length of treatment
• For a woman of childbearing potential:
• Negative blood pregnancy test at the inclusion visit
• Patient accepting highly effective contraception for the duration of study participation and 182 days after discontinuation of the study or treatment.
• Initiation of HU treatment in a patient requiring intensification of therapy in the context of sickle cell disease
• Patient hospitalised (e.g. vaso-occlusive crisis) and/or whose HU treatment is not balanced (MTD not reached)
• Informed consent signed, as appropriate, by :
  • The patient and/or
  • The holder(s) of parental authority and the minor subject if capable of discernment
• Subject affiliated to a social health insurance scheme or beneficiary
• Subject who has been informed of the results of the prior medical examination, and/or of whom the holder(s) of parental authority has (have) been informed
• Subject able to understand the objectives and risks of the research and to give dated and signed informed consent

Exclusion criteria:

• HU patient who has achieved MTD (based on haematological criteria) or is not therapeutically ineffective or hydroxyurea dosage > 35 mg/kg/day.
• Refusal to accept the use of a highly effective contraceptive method as defined during HU treatment and for 182 days for females and 92 days for males following such treatment (fertile patients only)
• Patient with a parental plan within 18 months
• Hypersensitivity to the active substance or to any of the excipients of the medicinal product
• Severe hepatic impairment
• Severe renal insufficiency
• Toxic signs of myelosuppression
• Neutrophils < 1500/mm3
• Platelets < 80 000/mm3
• Haemoglobin < 4.5 g/dL
• Reticulocytes < 80,000/mm3 if haemoglobin concentration < 9 g/dL
• Patient with transfusion, exchange transfusion or erythropoietin administration within 3 months of inclusion
• Patient with HIV
• Unable to give subject informed information (subject in emergency situation)
• Inability of the subject to undergo the medical follow-up of the trial for geographical, social or psychological reasons
• Subject under guardianship or curatorship
• Pregnancy or breastfeeding in the case of adolescents or adults
• Subject in an exclusion period (determined by a previous or ongoing study)
• Concurrent inclusion in another drug study
• Subject under court protection