Overview
The aim of this project is to investigate the presence of bacteria in the gallbladder wall and the bile in patients undergoing cholecystectomy, to determine if the standard empirical antibiotic treatment used currently is effective against the most common pathogens.
Description
The investigators will compare two groups of patients; patients with acute calculous cholecystitis undergoing acute laparoscopic cholecystectomy and patients without cholecystitis undergoing elective laparoscopic cholecystectomy.
Secondly, the investigators wish to investigate if the presence of bacteria correlates with the grade of inflammation, and whether this can predict when cholecystitis is caused by infection.
In a clinical setting, this can provide important knowledge regarding the choice of antibiotics currently used to treat and prevent infection in the gallbladder.
The investigators will conduct two studies, one investigating the bacteria in bile and in the gallbladder wall and the resistens of the bacteria to antibiotics and another study investigating the histopathology of the gallbladder wall to see if the bacteria present is the cause of inflammation or an actual infection.
Eligibility
Inclusion Criteria:
- Age above 18 years
- Signed consent form
- Patients having cholecystectomy performed at the Department of Surgery, Sygehus Lillebælt, Kolding.
Exclusion Criteria:
- Administration of any type of antibiotics one month prior to surgery in the control group.
- Previous instrumentation of the bileducts
- Previous clinical acute cholecystitis treated conservatively with antibiotics
Patients will be precluded from the studypopulation in cases of pPerioperative perforation
of the gallbladder, or conversion to open cholecystectomy or in cases of failed attempts to
extract bile or samples from the gallbladder wall.