Overview

A Phase 2a Multi-Center, Prospective, Randomized, Controlled Study to Evaluate the Safety and Efficacy of Topically Applied PEP-TISSEEL in Subjects with Diabetic Foot Ulcers (DFU)

Eligibility

Inclusion Criteria:

1. Males and females ≥ 18 years of age
2. Properly obtained written informed consent
3. Documented history of Type I or Type II Diabetes Mellitus, requiring oral and/or insulin replacement therapy
4. The index ulcer is classified as Wagner grade 1 ulcer and remains Wagner 1 Grade between Screening and Randomization/Baseline visit (Visit 1 through Visit 3)
5. These ulcers are superficial, full-thickness ulcers limited to the dermis, not extending to the subcutaneous tissue
6. Area of index ulcer must be between 1 cm2 to 15 cm2 post debridement at screening and baseline
7. The index ulcer must be located anatomically on the foot with ≥ 50% of the wound area below the medial or lateral malleolus
8. Presence of a persistent nonhealing DFU for at least 4 weeks from Randomization and not more than 1 year that has failed to respond to SOC at any point during this timeframe
9. Adequate vascular perfusion as evidenced by one of the following:
   1. Dorsal transcutaneous oxygen measurement (TCOM/TcPO2) measurement of

      ≥ 40 mmHg within 90 days of Screening (Visit 1 or 2)

   2. Ankle Branchial Index (ABI) between 0.7 and 1.3 within 90 days of Screening (Visit 1 or 2) using the extremity on which the index ulcer is located
   3. Arterial Doppler ultrasound evaluating for biphasic or triphasic dorsalis pedis and/or posterior tibial vessels at the level of the ankle or a TBI (Toe Brachial Index) of > 0.6 is acceptable within 90 days of Screening (Visit 1 or 2)
10. The index ulcer has been offloaded with protocol defined offloading device during

    Screening (Run-In) period through Randomization/Baseline visit.

11. Must meet one of the following criteria:
    1. Female subjects of Non-Child-Bearing Potential defined as: i. Postmenopausal for at least 1 year (Subject verbal confirmation acceptable), or surgically sterilized (i.e., hysterectomy or bilateral oophorectomy more than 3 months prior to Screening (Visit 1 or 2)), or ii. Bilateral tubal ligation more than 6 months prior to Screening (Visit 1 or 2), or iii. Must have a negative serum β-hCG pregnancy test at Visit 1 and not be breastfeeding prior to being administered with the study drug b. Male subjects of Non-Childbearing Potential are defined as those vasectomized subjects whose vasectomy was performed 6 months prior to Screening (Visit 1 or 2) or those diagnosed as sterile by a physician c. Females and Males of Childbearing Potential who practice an acceptable method of contraception defined as the use of any form of hormonal contraceptive, a barrier method with spermicide, condoms, intrauterine device, or abstinence from sexual intercourse starting at least 60 days prior to Screening and continuing at least 30 days following the last treatment.

             Female will undergo a negative serum β-hCG pregnancy test at Visit 1 and an additional
             urine test at Baseline/Randomization (Visit 3 or Day 0) and must not be breastfeeding
             prior to being administered with the study drug
         12. Ability to comply with the study protocol as per investigator discretion
        Exclusion Criteria:
          1. Actively undergoing chemotherapy treatment (localized radiation treatment is allowed
             if it is not on the DFU wound site and in remission based on scans, bloodwork or some
             other kind of test, such as a breast biopsy or a bone marrow biopsy)
          2. Ulceration with exposed tendon, capsule, or bone
          3. Suspicion of bone or joint infection by clinical or other criteria as per STONEES
             criteria below:
             a. STONEES criteria for infection: i. size increase, ii. temperature elevation, iii.
             os (probe to bone) iv. new areas of breakdown v. exudative vi. erythema/edema vii.
             smell
          4. Unable or unwilling to utilize the protocol defined offloading device
          5. Subjects who have undergone endovascular or open revascularization of the index limb
             within the last 30 days from Screening.
          6. Index ulcer has decreased in area by ≥ 30% between Screening (Visit 1) and Baseline
             visits
          7. Any subject that is currently on/or requires oral, systemic or topical antibiotics, or
             is anticipated to require their use during the course of the study
          8. Any subject that has vascular compromise requiring surgical intervention or has
             undergone vascular reconstruction or angioplasty less than 1 month prior to
             randomization. Any planned surgical procedures during the study participation
          9. Serum Creatinine level > 3.0 mg/dL
         10. Hemoglobin A1c (HbA1c) >12%
         11. Aspartate Aminotransferase (AST, GOT) and/or Alanine Aminotransferase (ALT, GPT) >3x
             the upper limit of normal
         12. Acute active Charcot foot
         13. The location of the index ulcer is within 2 cm of any other ulcer
         14. Any subject that would be unable to safely monitor the infection status of the index
             ulcer at home and return for scheduled visits
         15. History of immunosuppression or taking immunosuppressive agents including systemic
             corticosteroids, except stable daily doses of 5 mg/day or less for chronic conditions
             for up to 5 days
         16. Any subject with a life expectancy ≤ 6 months
         17. Pregnancy, including a positive pregnancy test at Baseline, or lactation/breastfeeding
             at anytime
         18. Use of investigational drugs or biologics within 28 days prior to screening (Visit 1
             or 2)
         19. History of a concurrent condition that, in the Investigator's opinion, would
             jeopardize the safety of the subject or compliance with the protocol
         20. Known or suspected active abuse of alcohol, or non-prescription drugs
         21. Participation in another interventional clinical study or study in the past 30 days of
             Screening (Visit 1 or 2) or concurrent participation in another interventional
             clinical study
         22. Subjects who have untreated Hep C
         23. Subjects who are HIV positive
         24. Subjects on anticoagulation that are not maintained within the International
             Normalized Ratio (INR) of > 3.0