Overview

The goal of this interventional study is to create hepatocellular carcinoma organoids from liver bioptic samples of individuals with hepatocellular carcinoma. The main questions it aims to answer are:

• the feasibility of hepatocellular carcinoma organoids integrated with host gut microbiota and peripheral blood mononuclear cells
• the molecular pattern of the organoid tumor microenvironment
• the in vitro therapeutic response of hepatocellular carcinoma organoids

Eligibility

Inclusion Criteria:

• Ability to express informed consent
• Patients ≥ 18 years of age
• Radiological diagnosis of hepatocellular carcinoma (HCC)

Exclusion Criteria:

• Patients <18 years of age
• Contraindications for liver biopsy: ascites, platelet count <50.000, International Normalized Ratio (INR) >1.7
• Active viral infection
• Refusal to give informed consent for the study