Overview
Compare the bacterial digestive microbiota during the stay in neonatal intensive care between a group of premature newborns developing a NEC (necrotizing enterocolitis) and a group of newborns free from NEC.
Description
Monitoring of a cohort of premature newborns with constitution of a collection of biological samples.
follow-up of newborns from birth up to 37 weeks of amenorrhea. The medical care of children will not be affected by their participation in the research.
- Sampling of amniotic fluid at the time of rupture of the water bag
- Sampling of the blood contained in the umbilical cord after clamping and section of the cord
- Sampling of meconium during the first 24 hours of life in intensive care / neonatal / maternity ward
- Collection of a daily stool sample until the end of hospitalization and when a NEC occurs
- Sampling of gastric residues during an episode of NEC
- Taking a daily sample of the newborn's enteral food
- Sampling of 500 µL of additional blood weekly during the punctures made for the treatment
- Daily statement of constants
- Record of all complications related to prematurity (HIV, bronchodysplastic, sepsis)
- Blood analysis: CBC, platelets and CRP (data analyzed as part of the treatment). Specific analyzes for research will assess: the level of tryptase, IL4 and TLR4. The samples will be stored and analyzed in the immunology laboratory of the CHU Clermont Ferrand (Pr B. Evrard)
- Analysis of the meconium sample and each stool emitted during hospitalization, and during an episode of ECUN
Eligibility
Inclusion Criteria:
- born prematurely < 37 weeks of amenorrhea at the maternity ward of the CHU
- affiliated with a social security regime
- the holders of parental authority are able to give an informed consent to participate in the research
Exclusion Criteria:
- fetal pathology
- newborns whose holders of parental authority are under guardianship or under the age of 18
- refusal of participation by holders of parental authority