Overview
In this study we plan to demonstrate that ethanol induces skeletal muscle autophagy to degrade MAA adducts.
Description
Hypothesis: Ethanol mediated modification of skeletal muscle proteins to form MAA adducts that in turn induce skeletal muscle autophagy.
Patients with alcoholic steatosis, hepatitis and cirrhosis (n=10 each) will be recruited from the liver transplant nutrition clinic or the hepatology inpatient service and their body composition quantified using anthropometry, bioelectrical impedance analysis, CT image analysis and DEXA if available.
Control Subjects. Controls will be recruited by advertisement. All control subjects will have a normal clinical history, physical examination, and screening chemistries. They will not have any significant medical conditions requiring the use of medications. Their nutritional status within 20% of normal as defined by ideal body weight.
Eligibility
Inclusion Criteria:
Alcoholic liver disease:
- clinical, biochemical, imaging criteria and liver biopsy where available
- Age 18 - 65 years old
- Controls
- Serum liver transaminases (i.e. ALT and AST) 40 IU/L
- Normal liver ultrasound
- Age 18 - 65 years old
Exclusion Criteria:
For both groups - alcoholic liver disease and controls:
- Poorly controlled diabetes mellitus (HbA1C>9.5 g/dl)
- Untreated Hyper- / hypo- thyroidism
- Patients on dialysis, renal disease with serum creatinine 1.5 mg/dL
- Active intravenous drug use
- History of bowel surgery or gastric bypass surgery
- Medications known to alter muscle protein metabolism (i.e. corticosteroids, tamoxifen, high-dose estrogen, testosterone or anabolic steroids)
- Metastatic disease, Advanced cardiac or pulmonary disease
- Pregnancy
- Coagulopathy- INR >1.4 and platelet count <80,000/ml.