Overview

The goal of this observational study is to learn about the key bacterial flora and metabolites associated with appendicitis in children. The main questions it aims to answer

are

• To screen out the key biomarkers of pediatric appendicitis.
• What are the microbial differences in different parts of pediatric appendicitis patients.

Participants will detect feces using 16s ribosomal RiboNucleicAcid (16S rRNA) gene sequencing technology and differences of the fecal metabolites between healthy children and appendicitis children were analyzed using untargeted metabolomics based on Liquid chromatography-tandem mass spectrometry(LC-M S/MS) platform.Through the analysis of intestinal bacterial flora and metabolomics association and the differential analysis of intestinal bacterial flora in different parts of the case group, the key bacterial flora and metabolites were excavated.

Eligibility

Inclusion Criteria:

• Children aged 1-14 years who were initially diagnosed with appendicitis and underwent laparoscopic appendectomy
• Children aged 1-14 years with non-digestive tract disease and no history of digestive tract disease

Exclusion Criteria:

• A family history of intestinal disease
• Allergic to antibiotic therapy drugs
• Patients with long-term drug use causing microbial structure changes or intestinal flora disorders
• Patients with other congenital diseases such as Meckel's diverticulum, intestinal rotation and other malrotation
• With other systemic diseases, such as obvious upper respiratory tract infection or myocarditis
• Children with other digestive system diseases after auxiliary examination or surgical diagnosis