A Phase Ib Trial of Azacitidine, Venetoclax and Allogeneic NK Cells for Acute Myeloid Leukemia (ADVENT-AML)

Overview

To learn if adding a healthy person's natural killer (NK) cells to the combination of Azacitidine and Venetoclax can help to control AML. NK cells are cancer- and infection-fighting immune cells.

Description

Primary Objective:

-To evaluate Safety of the combination of azacitidine, venetoclax and allogeneic NK cells

Secondary Objective:

• To estimate overall response rate (ORR)
• To estimate rate of CR/CRi by 4 cycles of therapy
• To estimate rate of MRD negative by 6 cycles
• To estimate overall survival (OS)
• To estimate relapse-free survival (RFS)

Exploratory Objectives:

• To estimate rate of CRc MRD negative at any time
• To estimate event-free survival (EFS)
• To estimate duration of response (DOR)
• To estimate median time to blood count recovery
• To estimate median time to first response
• To estimate median time to negative MRD
• To evaluate persistence of NK cells in peripheral blood
• To explore biomarker of response and resistance
• Using propensity-score matching analysis (per post-hoc plan) to explore the difference in response, survival adverse events in contemporary historical control population treated with HMA-VEN

Eligibility

Inclusion Criteria:

1. Patients need to have a confirmed diagnosis of AML, or MDS/AML with 10% to 19% blasts, per the International Consensus Classification 2022 or the WHO 2022 classification.43,44

   Dose escalation cohort:

2. Patients ≥18 years with R/R AML or R/R MDS/AML, other than acute promyelocytic leukemia (APL), or core binding factor (CBF) AML with no available standard treatment options.
3. Relapsed or refractory disease defined by standard criteria as follows
   1. Relapsed: Bone marrow blasts ≥5%, reappearance of blasts in the blood, or development of extramedullary disease following achievement of CR/CRi/MLFS b. Refractory: Failure to achieve CR/CRi/MLFS following initial treatment, with evidence of persistent leukemia by blood and/or bone marrow evaluation c. Appropriate prior therapy in order for patient to be deemed relapsed or refractory include the following
   2. 7+3 based induction: 2 cycles of for patients <60 years, and 1 cycle for patients who are either ≥60 years or unfit for intensive therapy ii. 1 cycle of induction regimen containing intermediate dose or higher of cytarabine iii. 2 cycles of venetoclax with HMA/LDAC +/- other agents iv. 4 cycles of HMA alone d. For patients in first relapse, the dose escalation cohort will only enroll patients in early first relapse, i.e., first remission duration of ≤12 months.
4. Patients relapsing after allo-SCT may be eligible if they have recovered from all

   transplant-related toxicities and are off all immunosuppression, with no more than grade 1 chronic GVHD. Physiologic dose of steroids (≤10 mg prednisone or equivalent) may be acceptable.

5. Patients with actionable mutations with available FDA-approved therapies, e.g., FLT3, IDH1/2 inhibitors may be enrolled after they have exhausted such available FDA approved treatment options.

Dose expansion cohort:

        Dose expansion cohort will only enroll older/unfit patients with newly diagnosed adverse
        risk AML or MDS/AML who are ineligible for intensive chemotherapy and/or are ineligible for
        or decline to receive allo-SCT.
        6. Adverse risk AML or MDS/AML defined per AML ELN 2022 recommendations.
        7. Age ≥ 75 years, or
        8. Age ≥ 18 years with at least one of the following comorbidities
          1. ECOG PS 2 or 3
          2. Left ventricular ejection fraction (LVEF) ≤ 50%
          3. Lung diffusing capacity for carbon monoxide (DLCO) ≤ 65% of expected
          4. Forced expiratory volume in 1 second (FEV1) ≤ 65% of expected
          5. Chronic stable angina or congestive heart failure controlled with medication
          6. Other comorbidity or conditions that the Investigator judges as incompatible with
             intensive chemotherapy, or allo-SCT which must be documented
             9. Patients with antecedent aplastic anemia, myelodysplastic syndrome, or chronic
             myelomonocytic leukemia may be eligible if they had not received prior hypomethylating
             agent, BCL2 inhibitors, MCL1 inhibitors, chemotherapy (definitive or therapeutic
             intent), or allo-SCT for MDS. Acceptable prior therapies include erythropoietin
             stimulating agents, thrombopoietin receptor agonists, lenalidomide, luspatarcept,
             anti-thymocyte globulin, cyclosporine, and iron chelating agents.
             All patients:
             10. Adequate hepatic function (direct bilirubin ≤ 2 x upper limit of normal (ULN)
             unless increase is due to Gilbert's disease or leukemic involvement, and AST and/or
             ALT ≤ 2.5 x ULN unless considered due to leukemic involvement, in which case direct
             bilirubin or AST and/or ALT ≤ 3 x ULN will be considered eligible.)
             11. Adequate renal function with creatinine clearance ≥ 45 mL/min calculated by the
             Cockcroft-Gault formula or measured by 24-hour urine collection
             12. The effects of these agents on the developing human fetus are unknown. For this
             reason, and because other therapeutic agents used in this trial may be teratogenic,
             women of childbearing potential and men must agree to use adequate contraception
             (hormonal or barrier method of birth control; abstinence) prior to study entry, for
             the duration of study participation, and for at least 90 days after last treatment.
             This includes all female patients between the onset of menses (as early as 8 years of
             age) and 55 years unless the patient presents with an applicable exclusionary factor
             which may be one of the following:
               -  Postmenopausal (no menses in greater than or equal to 12 consecutive months).
               -  History of hysterectomy or bilateral salpingo-oophorectomy.
               -  Ovarian failure (follicle-stimulating hormone and estradiol in menopausal range,
                  who have received whole pelvic radiation therapy).
               -  History of bilateral tubal ligation or another surgical sterilization procedure.
                  13. Approved methods of birth control are as follows: Hormonal contraception
                  (i.e., birth control pills, injection, implant, transdermal patch, vaginal ring),
                  Intrauterine device (IUD), tubal ligation or hysterectomy, subject/partner post
                  vasectomy, implantable or injectable contraceptives, and condoms plus spermicide.
                  Not engaging in sexual activity for the total duration of the trial and the drug
                  washout period is an acceptable practice; however, periodic abstinence, the
                  rhythm method, and the withdrawal method are not acceptable methods of birth
                  control. Should a woman become pregnant or suspect she is pregnant while she or
                  her partner is participating in this study, she should inform her treating
                  physician immediately.
                  14. Men treated or enrolled on this protocol must also agree to use adequate
                  contraception prior to the study, for the duration of study participation, and 4
                  months after completion of treatment.
                  15. Ability to understand and the willingness to sign a written informed consent
                  document.
             Exclusion Criteria:
             1. Patients with t(15;17), t(8;21), inv(16), or t(16;16) karyotypic abnormality
             16. Patient has a white blood cell count > 15 x 10⁹/L. Hydroxyurea, and/or cytarabine
             (up to 2 g/m2 total) used as supportive care is permitted to meet this criterion.
             17. Patients who have received high-dose (e.g., > 10 mg prednisone or equivalent)
             systemic steroid therapy or any other form of immunosuppressive therapy within 1 week
             or 5 half-lives of first NK cell infusion date (cycle 1 Day 8), whichever is longer.
             18. Patients with known symptomatic or uncontrolled CNS leukemia. 19. Patient has
             systemic fungal, bacterial, viral or other infection that is exhibiting ongoing
             signs/symptoms related to the infection without improvement despite appropriate
             treatment.
             20. Any signs or symptoms of active CNS pathology within 6 months of screening
             including history of seizures requiring anti-epileptics, focal neurological deficit,
             stroke, dementia, brain injury, or organic brain pathology. Any subarachnoid
             hemorrhage or CNS bleed within 3 months of screening.
             21. Patients with any severe gastrointestinal or metabolic condition which could
             interfere with the absorption of oral study medications as determined by the
             investigator.
             22. Active and uncontrolled comorbidities including decompensated congestive heart
             failure NYHA class III/IV, clinically significant and uncontrolled arrhythmia as
             judged by the treating physician.
             23. Known active hepatitis B (HBV) or Hepatitis C (HCV) infection with detectable
             viral DNA or RNA, respectively, or known HIV infection.
             24. Corrected QT interval (QTc) > 480 msec or history of Torsades de pointes
             25. Any other medical, psychological, or social condition that may interfere with
             study participation or compliance, or compromise patient safety in the opinion of the
             investigator.
             26. Any previous or concomitant malignancy, except when the patient has completed
             definitive curative-intent treatment with chemotherapy and/or surgery and/or
             radiotherapy at least 3 months prior to enrollment. Patients having completed
             definitive treatment for the following conditions may be eligible immediately after
             completion of definitive curative-intent therapy, after healing of wounds, and no
             evidence of residual disease by examination, imaging, and/or cytology/pathology, e.g.,
             non-melanoma skin cancers, or a carcinoma in-situ, e.g., ductal carcinoma in situ,
             urothelial cancer, cervical cancer, localized prostate cancer, pre-cancerous colon
             polyp, etc.
             27. Weight <50 kg
             28. Major surgery within 4 weeks prior to screening or a major wound that has not
             fully healed.
             29. Patients under legal protection measure (guardianship, trusteeship or safeguard of
             justice) and/or uncontrolled psychiatric comorbidities, ongoing illicit substance
             abuse, inability, any impairment or unwillingness to comply with the treatments,
             follow-up, requirements and procedures of this clinical trial.
             30. A known hypersensitivity or severe allergy to study drug components or diluents
             31. Nursing women, women of childbearing potential (WOCBP) with positive urine or
             serum pregnancy test, or WOCBP who are not willing to maintain adequate contraception
             32. Pregnant women are excluded from this study because study agents may have the
             potential for teratogenic or abortifacient effects. Because there is an unknown but
             potential risk for adverse events (AEs) in nursing infants secondary to treatment of
             the mother with study agents, breastfeeding should be discontinued if the mother is
             treated on this study.