Overview
The goal of this observational study is to investigate the alterations in gut microbiota and metabolites among patients with uric acid stones following the administration of potassium sodium hydrogen citrate. The main question it aims to predict the potential metabolic mechanism and therapeutic target of potassium sodium hydrogen citrate in treating uric acid stones through analysis of gut microbiota and metabolomics. The participants were required to undergo a 3-month drug intervention, providing blood, urine, and stool samples before and after treatment. No additional interventions were implemented for the subjects.
Description
The objectives of this study were to investigate the association between the presence of bacterial genera and short-chain fatty acids (SCFAs) in stool, as well as biochemical elements in blood and urine, among patients with uric acid nephrolithiasis. The sensitivity difference of potassium sodium hydrogen citrate in treating uric acid kidney stones was examined through gut microbiota analysis and metabolomics to predict potential metabolic mechanisms and sensitive targets for treatment. Blood biochemistry, 24-hour urine composition analysis, and other indicators were collected from the subjects. Fecal samples were obtained for 16S ribosomal RNA sequencing to analyze the characteristics of gut microbiota in relation to blood and urine biochemical metabolism indicators. Subjects received treatment with potassium sodium hydrogen citrate granules for a duration of 3 months. Blood and urine biochemical indexes, fecal samples, 16S ribosomal RNA sequencing data, and short-chain fatty acid levels in fecal samples were collected before and after treatment. The care of the enrolled patients will not be subject to any intervention.
Eligibility
Inclusion Criteria:
All stone patients were diagnosed using urologic ultrasonography, kidney-ureter-bladder
(KUB) X-ray, or abdominal computed tomography (CT). Stone samples were obtained through
ureteroscopic lithotripsy (URSL), percutaneous nephrolithotomy (PCNL), or extracorporeal
shock wave lithotripsy (ESWL). Stones were analyzed using an automated infrared
spectroscopy system, LIIR-20 (Lanmode Scientific Instrument Co., Ltd., Tianjin, China), and
the main components were determined based on the most abundant substances listed in the
report, which were classified as pure or mixed uric acid stones (anhydrous uric acid
content >50%).
Exclusion Criteria:
Patients with malignancy, chronic liver insufficiency, a history of statin use, and thyroid
or parathyroid disease were excluded from the study. Similarly, individuals with a history
of urolithiasis or dyslipidemia as well as those who had used statins were excluded from
the control group. Participants were also excluded if they had taken antibiotics or immune
suppressants within one month prior to fecal sampling, or had a history of chronic diarrhea
or constipation, chronic enteritis, irritable bowel syndrome, gastrointestinal tumors or
intestinal surgery.