Overview

The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a [2H5]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.

Eligibility

Inclusion Criteria:

• Healthy, recreationally active, male and female participants.
• Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
• Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
• Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
• Participants will be aged 18-35 years old.
• Participants must be normal to overweight (e.g., BMI 18.5-30).
• Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
• Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).

Exclusion Criteria:

• Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
• Self-reported regular tobacco use.
• Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
• Regular use of anti-inflammatory drugs (e.g., ibuprofen).
• Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
• Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
• Use of birth control and discontinued use in the last 3-months (female only).