Overview
The primary objective of this study will be to determine the influence of a presleep vitamin C-enriched, collagen-modelled supplement (CVC) on the metabolic fate of dietary glycine using a [2H5]glycine tracer within the intramuscular connective tissue at rest and after a bout of resistance exercise. Other outcomes will be related to regulation collagen remodelling in skeletal muscle.
Eligibility
Inclusion Criteria:
- Healthy, recreationally active, male and female participants.
- Healthy will be defined as someone who does not have a health condition that would compromise their ability to safely participate in the strenuous physical activity involved in our study as screened for by the Get Active Questionnaire.
- Willing to abstain from resistance exercise for 2 weeks prior to the first metabolic trial and for the duration of the study.
- Participants must be able to abstain from lower-body resistance and/or plyometric exercise for at least 2 weeks prior to the first metabolic trial.
- Participants will be aged 18-35 years old.
- Participants must be normal to overweight (e.g., BMI 18.5-30).
- Participants are willing to abide by the compliance rules of this study (e.g., abstain from physical activity and alcohol 48h prior to each trial).
- Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
Exclusion Criteria:
- Inability to adhere to any of the compliance rules judged by principal investigator or medical doctor.
- Self-reported regular tobacco use.
- Self-reported illicit drug use (e.g. growth hormone, testosterone, etc.).
- Regular use of anti-inflammatory drugs (e.g., ibuprofen).
- Self-reported regularly sleeping less than an average of 7 hours over 3 days (minimum recommended daily amount).
- Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- Use of birth control and discontinued use in the last 3-months (female only).