Overview

This study is a single-center exploratory clinical trial. It is estimated that 9-24 subjects will be enrolled. The "3+3" dose escalation design is adopted. The main purpose is to evaluate the safety of RD133 in the treatment of subjects with relapsed or refractory MSLN-positive solid tumors and explore the Recommend phase II dose of RD133 in the treatment of patients with relapsed/refractory MSLN-positive solid tumors.

Eligibility

Inclusion Criteria:

1. The subject must personally sign the written informed consent form approved by the ethics committee before the start of the study;
2. ≥18 years of age;
3. Have received at least 2 prior standard treatments, and achieved no response to the last-line treatment;
4. >25% Mesothelin positive rate on tumor cell membrane confirmed by prior immunohistochemistry of tumor tissue or freshly punctured tissue;
5. Expected survival ≥ 12 weeks;
6. ECOG score ≤ 2;
7. At least one measurable target lesion that meets the RECIST v1.1 standard;
8. Female or male subjects with fertility should agree to practice an effective method of contraception from the day of signing the ICF until 365 days after the infusion. Effective method of contraception is defined as: abstinence or contraceptive methods with an annual failure rate of <1% specified in the plan. ；
9. Before being enrolled in the group, the subject must have proper organ function and meet all of the following criteria:

        9.1 The absolute value of neutrophils≥1.0×10^9/L (granulocyte colony stimulating factor
        (G-CSF) support is allowed, but must be without supportive treatment within 7 days before
        the examination); 9.2 Platelet count ≥75×10^9/L (must be without blood transfusion support
        [including blood component transfusion] or thrombopoietin [TPO], or other treatments for
        the purpose of increasing platelets within 7 days before the examination); 9.3 Hemoglobin
        ≥9 g/dl (must be without blood transfusion support [including blood component transfusion]
        within 7 days before the examination); 9.4 Bilirubin value ≤1.5×upper limit of normal (ULN)
        (except bile duct obstruction caused by tumor compression); 9.5 Creatinine clearance rate
        ≥60 ml/min; 9.6 ALT or AST ≤2.5×upper limit of normal (ULN) (with liver involvement
        ≤5×ULN); 9.7 The results of echocardiography indicate that the cardiac ejection fraction is
        ≥ 50%, without obvious pericardial effusion; 9.8 Stable coagulation function: INR ≤ 1.5,
        APTT ≤1.2×ULN (except tumor-related anticoagulation therapy); 9.9 >91% basic blood oxygen
        saturation in the natural indoor air environments.
        Exclusion Criteria:
          1. Subject who has received any of the following prior treatments:
             1.1 Subject with acute or chronic graft-versus-host disease (GVHD) who need systemic
             treatment within 4 weeks before enrollment; 1.2 Subject who has received gene therapy
             before enrollment; 1.3 Subject who needs systematic immunosuppressive therapy (except
             topical drugs) to control autoimmune diseases (eg: Crohn's disease, rheumatoid
             arthritis, systemic lupus erythematosus, etc.), immunodeficiency or other diseases in
             the first 2 years after enrollment; 1.4 Subject who has been injected with live
             vaccines within 4 weeks before enrollment; 1.5 Subject has received other
             interventional clinical research drugs within 12 weeks before apheresis.
          2. Subject with central metastasis or complete intestinal obstruction;
          3. Subject with moderate or more severe hydrothorax and ascites which are hard to control
             by conventional treatment and require continuous catheter drainage;
          4. With an active malignant tumor in the past 5 years, unless it is a curable tumor and
             has been obviously cured, such as basal or squamous cell carcinoma, cervical or breast
             carcinoma in situ, etc.
          5. Subject with positive hepatitis B surface antigen (HBsAg) or hepatitis B core antibody
             (HBcAb) and abnormal HBV DNA test results in peripheral blood (abnormal HBV DNA test
             results are defined as: HBV DNA quantitative level higher than the lower limit of the
             detection center or higher than normal range of the detection center; or qualitative
             HBV DNA test positive); Hepatitis C virus (HCV) antibody positive and peripheral blood
             hepatitis C virus (HCV) RNA positive; Human immunodeficiency virus (HIV) antibody
             positive; Cytomegalovirus ( CMV) DNA test positive; syphilis test RPR positive.
          6. With an uncontrollable active infection (except genitourinary system infection and
             upper respiratory tract infection <CTCAE Grade 2).
          7. Severe heart disease: including but not limited to unstable angina, myocardial
             infarction (within 6 months before screening), congestive heart failure (New York
             Heart Association [NYHA] classification ≥ grade 3), severe arrhythmia.
          8. Subject with hypertension that cannot be controlled by medication.
          9. The toxicity of previous treatment has not been relieved to baseline or ≤1 (NCI-CTCAE
             v5.0, except for hair loss and laboratory abnormalities without clinical
             significance).
         10. Major surgery within 2 weeks before enrollment, or has surgery planned during the time
             the subject is expected to be infused with RD133 or within 12 weeks after RD133
             infusion (except planned surgery under local anesthesia).
         11. Subject who has a solid organ transplant.
         12. Women who are pregnant or breastfeeding.
         13. Subject with previous central nervous system diseases (such as cerebral aneurysm,
             epilepsy, stroke, Alzheimer's disease, mental illness, etc.) or mental disorders.
         14. Other unstable systemic diseases judged by the investigator: including but not limited
             to severe liver, kidney, or metabolic diseases that require medication.
         15. Known to have life-threatening allergic reactions, hypersensitivity reactions or
             intolerances to RD133 cell preparations or its components.
         16. Subject with hemorrhage, severe thrombosis judged by the Investigator, or
             hereditary/acquired hemorrhage and severe thrombosis (including hemophilia,
             coagulopathy, thrombocytopenia, hypersplenism, etc.), or are receiving thrombolytic or
             anticoagulant.
         17. The researcher believes that other situations are not suitable for inclusion in the
             group.