Xenon MRI Pulm Hypertension

Overview

The overall objective outlined in this study is to determine how pulmonary vascular remodeling in PAH at a cellular and pathological level is associated with changes in gas exchange physiology and hemodynamics (monitored with 129Xe MRI/MRS) and how these signals change with disease progression or treatment.

Description

In aim 1, the study team will compare 129Xe MRI signatures to lung explant pathology, cellular identity from single-cell RNA sequencing, and cellular phenotypes in gas exchange defects to areas that are spared, which the team will hypothesize are exposed to lower levels of hemodynamic shear stress. This will be done by performing 129Xe MRI scans in fifteen subjects (cohort 1) with PAH awaiting a lung transplant, followed by a pathologic assessment (with usual histopathology and single cell RNA sequencing) of the subject's explanted lung after transplant. The study team expect that areas of proliferation and fibrosis will correlate with 129Xe MRI gas exchange and spectroscopic defects

In aim 3, The study team will test whether directly monitoring lung pathology with 129Xe MRI will provide additional prognostic information to standard-of-care clinical monitoring in 45 subjects (cohort 2). At 6-month follow-up appointments, standard-of-care assessments including labs, echocardiography, and six-minute walk distance and 129Xe MRI will be collected. The study team expect that In aim 1, the study team will compare 129Xe MRI signatures to lung explant pathology, cellular identity from single-cell RNA sequencing, and cellular phenotypes in gas exchange defects to areas that are spared, which the study team hypothesizes are exposed to lower levels of hemodynamic shear stress. This will be done by performing 129Xe MRI scans in fifteen subjects (cohort 1) with PAH awaiting a lung transplant, followed by a pathologic assessment (with usual histopathology and single-cell RNA sequencing) of the subject's explanted lung after transplant. The study team expect that areas of proliferation and fibrosis will correlate with 129Xe MRI gas exchange and spectroscopic defects

Eligibility

Inclusion Criteria of Cohort 1

        Subjects must meet all of the following inclusion criteria to be eligible for enrollment
        into the trial:
          1. Outpatients of either gender, Age 18-75
          2. Awaiting a lung transplant
          3. Diagnosis of precapillary PH (right heart catheterization demonstrating hemodynamic
             criteria of a mean pulmonary artery pressure (mPAP) ≥ 25 mmHg, pulmonary vascular
             resistance ≥ 3 WU, pulmonary capillary wedge pressure ≤ 15 mmHg) in the setting of
             Group 1 (PAH), 3 (PH due to chronic lung disease, 4 (PH due to pulmonary artery
             obstructions), or 5 (PH due to miscellaneous causes)
          4. Willing and giving informed consent and adhere to visit/protocol schedules (consent
             must be given before any study procedures are performed).
          5. Women of childbearing potential must have a negative urine pregnancy test before MRI
        Exclusion Criteria of Cohort 1
        Subjects presenting with any of the following will not be included in the trials:
          1. Moderate to severe heart disease (LVEF <45%, Severe LV hypertrophy, Moderate to severe
             valvular disease)
          2. PH due to schistosomiasis
          3. Active cancer
          4. Sickle cell anemia
          5. Prisoners and pregnant women will not be approached for the study
          6. Conditions that will prohibit MRI scanning (metal in eye, claustrophobia, inability to
             lie supine)
          7. Medical or psychological conditions which, in the opinion of the investigator, might
             create undue risk to the subject or interfere with the subject's ability to comply
             with the protocol requirements
        Inclusion Criteria of Cohort 2
        Subjects must meet all of the following inclusion criteria to be eligible for enrollment
        into the trial:
          1. Treatment naïve or treatment started within the last 3 months
          2. Outpatients of either gender, Age 18-75
          3. WHO functional class (FC) 2-3 symptoms with a diagnosis of group 1 PH (mean pulmonary
             artery pressures (mPAP) > 20 mmHg, pulmonary capillary wedge pressure (PCWP) ≤ 15mmHg
             and pulmonary vascular resistance (PVR) ≥3 WU)
          4. Willing and able to give informed consent and adhere to visit/protocol schedules
             (consent must be given before any study procedures are performed).
          5. Women of childbearing potential must have a negative urine pregnancy test before MRI
        Exclusion Criteria of Cohort 2
        Subjects presenting with any of the following will not be included in the trials:
          1. Sarcoidosis
          2. Active cancer
          3. Sickle cell anemia
          4. Liver disease (Childs-Pugh class C)
          5. Any conditions that prevent the performance of 129Xe MRI scans.
          6. Prisoners and pregnant women will not be approached for the study.
          7. Conditions that will prohibit MRI scanning (metal in eye, claustrophobia, inability to
             lie supine).
          8. Medical or psychological conditions which, in the opinion of the investigator, might
             create undue risk to the subject or interfere with the subject's ability to comply
             with the protocol requirements