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Immunohistochemical Evaluation of DNA Methylation and Histone Modification in H. Pylori Associated Gastric Carcinoma

Recruiting
30 - 100 years of age
Both
Phase N/A

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Overview

The goal of this cross-sectional study is to evaluate epigenetic modifications in gastric carcinogenesis, mainly in H. pylori gastritis vs gastric carcinoma. The main question[s] it aims to answer are:

  • How does H. pylori infection cause gastric cancer?
  • Can DNA methylation and other epigenetic changes predict and affect the development of gastric cancer? Formalin-fixed-paraffin-embedded tissue specimens will be obtained and reviewed for comparison between epigenetic changes in H. pylori gastritis and gastric adenocarcinoma.

Eligibility

Inclusion Criteria:

  • Resected specimens and endoscopic guided biopsies of intestinal type gastric carcinoma, H. Pylori associated gastritis, and non-H. Pylori gastritis with:
  • Available full clinical data: (age, sex, complaint, findings in clinical examination).
  • Full data of previous endoscopy and radiology.

Exclusion Criteria:

  • Cases of diffuse type gastric carcinoma.
  • Any case of gastric cancer, H. Pylori associated gastritis, non-H. Pylori gastritis, benign gastric lesions with missing clinical, endoscopic, radiologic or follow-up data.
  • Cases with no available H&E stained slides or formalin fixed paraffin embedded block.

Study details

Gastric Carcinoma

NCT05811546

Assiut University

26 January 2024

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