Description

1. Retrospective review of old patients
   • Analysis of prediction factors for concomitant chemo-radiotherapy in locally advanced and advanced esophageal cancer from 2005 to 2015

Study Group

1. non responder
2. CR but recurrence
3. CR and survival loner than 2 years
4. Prospective case-control study: RNA sequencing & miRNA microarray
   • naive esophageal cancer patients who have a plan to receive concomitant chemo-radiotherapy --> acquirement of tissue, blood, clinical information --> isolation of RNA, DNA, serum database of clinical & demographic information

study group

1. non-responder (N=14)
2. Complete Remission (N=14)

For considering the drop rate, 2-fold screening

***Analysis of mRNA sequencing / miRNA sequencing**** first analysis of RNA sequencing: bioinformatician second analysis of RNA sequencing: principle investigator

3) Experimental set for qPCR (esophageal tissue) study group

1. non-responder (N=40)
2. complete remission (N=40)
3. TMA for screened exon gene
4. validation set for qPCR (esophageal tissue) new esophageal cancer set
5. non-responder (N=30)
6. complete remission (N=30)
7. follow-up of circulating miRNA in blood (baseline blood & post-CCRT blood 3M, 6M, 12M)
8. Prediction model for CCRT response in esophageal Cancer based on clinical and molecular factors

Test several combination models

1. gene + gene combination,
2. gene + clinical information combination,
3. gene + gene + clinical information combination
4. Validate the prediction model.
5. primary organoid culture and prediction of chemoradiotherapy response