Overview
The goal of this study is to understand how exogenous kisspeptin affects metabolism by evaluating responses to an oral glucose tolerance test
Description
Assignment: Each study subject will serve as their own control. The order of the visits will be randomized.
Delivery of Interventions:
- Prior to the study visit, subjects will undergo a review of their medical history and screening laboratories.
- During the inpatient study, the subjects will
- Undergo a 16-hour kisspeptin infusion
- Undergo an oral glucose tolerance test
Eligibility
Inclusion Criteria:
- History
-
- over the age of 18,
- normal pubertal development
- stable weight for previous three months,
- normal body mass index (BMI between 18.5-25)
- regular menstrual cycles
Physical examination:
• systolic BP < 140 mm Hg, diastolic < 90 mm Hg
Laboratory studies: (per MGH reference ranges)
- normal hemoglobin
- hemoglobin A1C < 6.5%
- BUN, creatinine not elevated
- AST, ALT < 3x upper limit of normal
Exclusion Criteria:
- active illicit drug use,
- history of a medication reaction requiring emergency medical care,
- difficulty with blood draws.
- history of chronic disease, except well controlled thyroid disease,
- recent use of prescription medications which interfere with metabolism or reproduction (recent = within 5 half-lives of the drug). Use of levothyroxine or seasonal allergy medications is acceptable,
- history of diabetes in a first degree relative,
- use of contraceptive pills, patches or vaginal rings within last 4 weeks.
- hyperlipidemia by fasting lipid panel
- positive serum pregnancy test (for all women)